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Vertical Autoclave

Product Overview

The Industrial-Grade Vertical Autoclave is a top-loading pressure steam sterilizer engineered for high-volume media preparation, glassware processing, and biohazard waste inactivation. Featuring a vertical cylindrical chamber layout, it optimizes laboratory floor space while accommodating tall Erlenmeyer flasks, deep culture bottles, and heavy-duty waste collection bins.
Equipped with microprocessor-managed thermal cycles and heavy-gauge stainless steel pressure vessels, the unit operates reliably in continuous multi-shift environments. It complies with international pressure vessel safety codes, serving research institutes, pharmaceutical QC labs, and clinical diagnostic facilities.

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Working Principle

Sterilization relies on forced saturated steam displacement under pressure to achieve thermal destruction of microbial cell walls, enzymes, and heat-resistant spores.

Air Evacuation:

Cold air pockets are eliminated via gravity displacement (steam forcing air downward through bottom drains) or mechanical pre-vacuum pulses (on select models) to ensure unobstructed steam penetration.

Thermal Penetration:

Saturated steam is injected into the sealed chamber, immediately condensing upon cooler load surfaces to transfer latent heat efficiently.

Hold Phase:

Internal chamber temperature is maintained at setpoint (typically 121 deg C at 0.10 MPa or 134 deg C at 0.22 MPa) for a validated holding time duration.

Exhaust and Drying:

Pressure is relieved through programmed venting profiles—slow exhaust for liquid media to prevent boiling-over, or active vacuum jacket drying for porous loads and glassware.

Key Features

Vertical Ergonomic Loading:

Deep cylindrical chamber layout minimizes operator lifting strain when handling heavy, fully loaded stainless steel stacking baskets.

Dynamic Microprocessor Control:

Features pre-programmed thermal cycles for liquids, wrapped and unwrapped instruments, and solid waste, supported by customizable user-defined profiles.

Automated Water Management:

Integrated water reservoir equipped with low-water level cutoff sensors to prevent heating element dry-firing and burnout.

Interlocked Safety Mechanism:

Dual mechanical and electronic pressure-temperature interlocks prevent lid opening while internal pressure exceeds 0.02 MPa or temperature remains above 80 deg C.

Traceability and Logging:

Built-in micro-printer and digital data ports export complete time, temperature, and pressure tracking curves for regulatory compliance audits.

Configuration and Components

Chamber Material: Seamless hydro-formed chamber constructed from AISI 304 or passivated AISI 316L stainless steel, offering superior resistance to chloride-induced stress corrosion cracking.

Heating Assembly: Dual heavy-duty Incoloy-sheathed immersion heating elements base-mounted for rapid thermal ramp-up and balanced heat distribution.

Valves and Seals: High-temperature-resistant, self-inflating silicone rubber gaskets and pneumatic solenoid control valves rated up to 140 deg C.

Pressure Vessel Safety: Integrated mechanical safety relief valve calibrated to factory maximum allowable working pressure (MAWP), backed by independent over-temperature cutoffs.

Exhaust Condenser: Built-in cooling coil condenses discharged steam prior to facility drainage, protecting plastic plumbing infrastructures.

Technical Specifications

Technical Parameter	Model VA-50	Model VA-75	Model VA-100
Chamber Capacity	50 Liters	75 Liters	100 Liters
Chamber Dimensions (Dia x Depth)	340 x 550 mm	400 x 600 mm	450 x 630 mm
Max. Working Temperature	134 deg C	134 deg C	138 deg C
Working Sterilization Pressure	0.22 MPa	0.22 MPa	0.23 MPa
Design / Max. Pressure	0.23 MPa	0.25 MPa	0.28 MPa
Chamber Material Grade	AISI 304 / 316L	AISI 304 / 316L	AISI 304 / 316L
Sterilization Timer Range	0 to 99 min	1 to 300 min	0 to 99 min
Power Supply	AC 220V, 3.0 kW	AC 380V, 3-phase, 4.6 kW	AC 380V, 3-phase, 6.5 kW

Applications

Microbiology and Life Sciences:

Preparation of nutrient agars, broths, and buffer solutions under sterile laboratory conditions.

Biohazard Containment Facilities:

Inactivation of infectious biological waste, contaminated petri dishes, pipette tips, and inoculation loops prior to municipal disposal.

Pharmaceutical Quality Control:

Sterilization of production glass vials, elastomer stoppers, and raw material testing components.

Clinical and Veterinary Diagnostics:

Thermal sterilization of surgical instrumentation and containment of bio-fluid waste.

Model Selection and Configuration Options

To match specific laboratory workflows, units are categorized into two baseline configurations:

Gravity Displacement Series (G-Series): Recommended for basic glassware, unwrapped metal instruments, and standard liquid media preparation where air pockets are easily cleared.

Pre-Vacuum Series (V-Series): Mandatory for facilities processing multi-layered biohazard waste bags, porous textile packs, or narrow-lumen hollow tubing requiring pre-cycle air extraction.

Modular Factory Options:
• Cooling Fan Acceleration: External cooling jackets reducing total batch turnaround time by up to 30 percent.
• Validation TC Port: 1.5-inch sanitary clamp port designed for thermal mapping probe insertion during site validation.
• Direct RO Water Feed Kit: Solenoid-controlled auto-filling mechanism connecting directly to facility purified water loops.

Manufacturing and Quality Control

01/

CNC Fabrication and TIG Welding: Chamber bodies undergo automated CNC laser cutting followed by specialized TIG root welding and internal surface passivating to eliminate micro-crevices.

02/

Hydrostatic Proof Testing: Every manufactured pressure vessel undergoes rigorous hydraulic testing at 1.5 times the maximum working pressure to confirm structural weld integrity.

03/

48-Hour Factory Burn-In: Completed units are subjected to continuous operational testing under simulated load profiles to verify thermal sensor accuracy and electrical safety loops.

04/

Regulatory Compliance: Built in strict adherence to ISO 9001 manufacturing standards; pressure vessels conform to CE / PED 2014/68/EU directives.

Customization and Integration

OEM and Private Labeling:

Chassis color customization, private logo embedding, and multilingual interface software packs (English, Spanish, French, German).

Validation Documentation Packages:

Complete factory test certificates, material traceability records, and IQ/OQ/PQ protocol documentation sets supplied for GMP-regulated environments.

Custom Stainless Racks:

Segmented wire baskets and deep lifting drums tailored to non-standard bioreactor vessel geometries.

FAQ

Q: What differentiates gravity displacement from pre-vacuum vertical autoclaves, and which is required for waste bags?

A: Gravity displacement relies on steam density to push lighter air downward through a bottom drain, which suits simple glassware and liquids. Biohazard waste bags trap dense pockets of air that block steam penetration; therefore, processing waste bags strictly requires a pre-vacuum model equipped with a mechanical vacuum pump to evacuate air prior to steam injection.

Q: Can raw municipal tap water be utilized, or is treated water mandatory?

A: Direct use of untreated tap water is strongly discouraged. High concentrations of calcium and magnesium minerals lead to rapid scaling on heating elements and pit corrosion on chamber walls. Facility operators must use distilled water or RO (Reverse Osmosis) water maintaining a conductivity of less than 15 uS/cm.

Q: How do safety interlocks prevent accidental opening under pressure?

A: A dual-monitoring system links the microprocessor control to physical pressure switches. The lid mechanism remains electronically and mechanically locked whenever internal chamber pressure exceeds 0.02 MPa or when internal fluid and air temperatures remain above 80 deg C, preventing sudden steam release and operator scalding.

Q: How does the system prevent liquid media from boiling over during exhaust?

A: When executing a liquid cycle, the controller overrides fast exhaust protocols, initiating a slow, controlled linear venting rate paired with natural thermal dissipation. This gradual depressurization prevents superheated media inside deep flasks from boiling over violently.

Q: What utility connections must be provisioned on-site prior to installation?

A: Utility requirements scale with chamber capacity:
Electrical: 50L units require single-phase 220V/50Hz (approx. 3.0 to 3.5 kW); 75L and 100L variants require 3-phase 380V power supplies (4.6 kW to 6.5 kW).
Water and Drain: A heat-resistant floor drain capable of handling effluent temperatures up to 90 deg C, alongside an optional pressurized RO water feed line.

Q: What compliance documentation is supplied with shipment?

A: Each autoclave includes a Certificate of Conformity, hydrostatic pressure test report, CE/PED documentation, and an operation manual. Comprehensive IQ/OQ/PQ protocol execution packages can be ordered alongside the unit for pharmaceutical and clinical validation compliance.

We're well-known as one of the leading vertical autoclave manufacturers and suppliers in China. With abundant experience, we warmly welcome you to buy high quality vertical autoclave for sale here from our factory. For price consultation, contact us.

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